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Cloning in biotechnology refers to processes used to create copies of DNA fragments, cells, the term also refers to the production of multiple copies of a product such as marley drugs generic viagra media or software. The term clone, invented by J. Haldane, is derived from the Ancient Greek word ╬╬¤╬ kl┼n, twig, referring to the process whereby a new plant can be created from a twig. In horticulture, the spelling clon was used until the twentieth century, since the term entered the popular lexicon in a more general context, the spelling clone has been used exclusively. In botany, the term lusus was traditionally used, Novaya viagra 2014 calendar is a natural form of reproduction that has allowed life forms to spread for more than 50 thousand years. It is the method used by plants, fungi, and bacteria.

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Cloning is commonly used to amplify DNA fragments containing whole genes and it is used in a wide array of biological experiments and practical applications ranging from genetic fingerprinting to large scale protein production. In practice, localization of the gene to a chromosome or genomic region does not necessarily enable one to isolate or amplify the relevant genomic sequence, initially, the DNA of interest needs to be isolated to provide a DNA segment of suitable size. Subsequently, a procedure is used where the amplified fragment is inserted into a vector. The vector is linearised using restriction enzymes, and incubated with the fragment of interest under appropriate conditions with an enzyme called DNA ligase, following ligation the vector with the insert of interest is transfected into cells.

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